source 15q ion exchange resin Search Results


ion exchange chromatography employed source15q resin  (Danaher Inc)
96
Danaher Inc ion exchange chromatography employed source15q resin
Ion Exchange Chromatography Employed Source15q Resin, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/source+15q+ion+exchange+resin/SOURCE+15Q/10__1074_slash_jbc__m116__747279-298-19-25
Average 96 stars, based on 1 article reviews
ion exchange chromatography employed source15q resin - by Bioz Stars, 2026-11
96/100 stars
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ion exchange chromatography  (Cytiva Europe)
96
Cytiva Europe ion exchange chromatography
Ion Exchange Chromatography, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/source+15q+ion+exchange+resin/SOURCE+15Q/ppr0319371-167-22-27
Average 96 stars, based on 1 article reviews
ion exchange chromatography - by Bioz Stars, 2026-11
96/100 stars
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recombinant profilin  (Cytiva Europe)
93
Cytiva Europe recombinant profilin
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Recombinant Profilin, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/source+15q+ion+exchange+resin/SOURCE+15Q+4%2E6%2F100+PE/pmc07911308-187-5-21
Average 93 stars, based on 1 article reviews
recombinant profilin - by Bioz Stars, 2026-11
93/100 stars
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ion-exchange chromatography source 15q  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc ion-exchange chromatography source 15q
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Ion Exchange Chromatography Source 15q, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/source+15q+ion+exchange+resin/ion+exchange+chromatography+source+15q/pm15723349-29-23-27
Average 90 stars, based on 1 article reviews
ion-exchange chromatography source 15q - by Bioz Stars, 2026-11
90/100 stars
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ion exchange  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc ion exchange
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Ion Exchange, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/source+15q+ion+exchange+resin/ion+exchange/pm17055435-226-19-23
Average 90 stars, based on 1 article reviews
ion exchange - by Bioz Stars, 2026-11
90/100 stars
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chromatography steps  (Cytiva Europe)
93
Cytiva Europe chromatography steps
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Chromatography Steps, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/source+15q+ion+exchange+resin/SOURCE+15Q+4%2E6%2F100+PE/pmc07911308-187-9-21
Average 93 stars, based on 1 article reviews
chromatography steps - by Bioz Stars, 2026-11
93/100 stars
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source 15 q ion-exchange column  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd source 15 q ion-exchange column
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Source 15 Q Ion Exchange Column, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/source+15q+ion+exchange+resin/15q+pe+4+6+100+anion+exchange+column/pmc00434390-42-13-18
Average 90 stars, based on 1 article reviews
source 15 q ion-exchange column - by Bioz Stars, 2026-11
90/100 stars
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ion exchange source 15q  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc ion exchange source 15q
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Ion Exchange Source 15q, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/source+15q+ion+exchange+resin/ion+exchange+source+15q/pm17055435-229-19-23
Average 90 stars, based on 1 article reviews
ion exchange source 15q - by Bioz Stars, 2026-11
90/100 stars
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source 15q  (Cytiva Europe)
96
Cytiva Europe source 15q
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Source 15q, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/source+15q+ion+exchange+resin/SOURCE+15Q/custom%4017094701%4022980642
Average 96 stars, based on 1 article reviews
source 15q - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier
ion-exchange chromatography  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc ion-exchange chromatography
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Ion Exchange Chromatography, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/source+15q+ion+exchange+resin/ion+exchange+chromatography/pm17529992-228-29-32
Average 90 stars, based on 1 article reviews
ion-exchange chromatography - by Bioz Stars, 2026-11
90/100 stars
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Image Search Results


Immunoblot with ( A ) recombinant L. albus profilin and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .

Journal: Nutrients

Article Title: Identification and Purification of Novel Low-Molecular-Weight Lupine Allergens as Components for Personalized Diagnostics

doi: 10.3390/nu13020409

Figure Lengend Snippet: Immunoblot with ( A ) recombinant L. albus profilin and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .

Article Snippet: Since the purification of the recombinant profilin via further chromatography steps (size exclusion chromatography and ion-exchange chromatography (source 15Q 4.6/100 PE, GE Healthcare, Uppsala, Sweden) were not successful, it was further purified via preparative SDS-PAGE.

Techniques: Western Blot, Recombinant, Negative Control, Inhibition, Incubation, Blocking Assay